Pure: Only 1 type of bacterium
Mixed: >1 type of bacteria in one culture
Contaminated: Unexpected occurrence of a bacterium in a cultureView
Culture Temps for Lab Experiments
25 oC -- Ambient, room temp
30 oC
37 oC -- Human body tempView
Nutrient Broth
Provides necessary carbon, nitrogen, and vitamins for most organisms used in the lab experimentsView
Enriched culture
For fastidious organisms; Trypticase soy agar (TSA) and blood agarView
Agar
A polysaccharide from marine (red) algae
Solid: 1.5% agar
Semi-solid: .5% agar
Forms of agar culture media: plate, slant, deep tubeView
Selective Media
Media containing a chemical to suppress undesired microbes (e.g., salt selects for Staphylococcus sp., bile salts select for enteric, gram- bacteria)View
Differential Media
Media designed to distinguish between species (e.g., lactose fermentation). Changes color if rxn occursView
Ubiquitous
The idea that microorganisms are present everywhere and at the same timeView